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If generating v2 sample sheets for instruments aside from those mentioned, verify the final sample sheet has the i5 index in the right orientation as explained higher than.
Explore how AI is reworking genomic exploration, driving medical insights, and enabling multiomic discovery
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The MiniSeq Speedy manufactured a recipe transform that takes advantage of the grafted oligo for the i5 index priming, so it's go through while in the ahead orientation just like a MiSeq.
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Illumina UD Indexes help sequencing of significantly much more samples per here operate with out compromising knowledge excellent.
This allows huge figures of libraries to get pooled and sequenced at the same time all through only one operate. In comparison to other indexing strategies, special twin indexing mitigates index hopping by filtering hopped reads.
The application instantly produces the reverse enhance as wanted for Examination. In the event the sequence is reverse complemented, this may only be revealed while in the SampleSheetUsed.csv file.
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Index 2: the i5 index column is inside the reverse complement orientation (are definitely the reverse enhance from the i5 indexes during the sample sheet).
Investigate how AI is reworking genomic exploration, driving scientific insights, and enabling multiomic discovery
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